Project
Part:BBa_K2944005:Design
Designed by: Davindra Singh Group: iGEM19_Concordia-Montreal (2019-10-16)
pGAL1-amilCP-tPGK1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The promoter needs to be inducible so that expression of protein depends on concentration of a transcription factor.
Source
The sequence of amilCP was obtained from a previous part (BBa_K592009) and the promoter and terminator are both obtained from the MoClo Toolkit.